Open AccessAntileishmanial effects of Sargassum vulgare products and prediction of trypanothione reductase inhibition by fucosterol
Author(s) -
Lauve Rachel Tchokouaha Yamthé,
Trudy Janice Philips,
Dorcas OseiSafo,
Paul Toukam Djouonzo,
Odame Agyapong,
Eunice Dotse,
Patrick Valère Tsouh Fokou,
Samuel K. Kwofie,
Fabrice Fekam Boyom,
Alexander Kwadwo Nyarko,
Regina Appiah–Opong,
Michael D. Wilson
Publication year - 2020
Publication title -
future drug discovery
Language(s) - English
Resource type - Journals
ISSN - 2631-3316
DOI - 10.4155/fdd-2020-0002
Subject(s) - amastigote , leishmania , leishmania donovani , sargassum , biochemistry , leishmania major , biology , chemistry , leishmaniasis , botany , visceral leishmaniasis , algae , parasite hosting , world wide web , computer science , immunology
Aim: To investigate the antileishmanial potency of Sargassum vulgare C. Agardh-derived products and the in silico inhibition of trypanothione reductase by fucosterol. Materials & methods: Sargassum vulgare crude extract and its derived fractions, subfractions and fucosterol were screened against Leishmania major and Leishmania donovani using the MTS and trypanothione reductase colorimetric assays. Macrophages viability was evaluated using the resazurin assay. The inhibition of trypanothione reductase by fucosterol was predicted in silico. Results: The crude extract, fractions 2, 4 and 7, subfractions 8.2 and 8.3 and fucosterol-exhibited antileishmanial activity on promastigote (IC 50 = 18.99–156.02 μg/ml), while fraction 1, subfraction 8.2 and fucosterol were active on L. major and L. donovani amastigote (IC 50 = 18.47–65.34 μg/ml) with low cytotoxicity. Interestingly, fucosterol showed the best activity against both parasites (IC 50 = 18.47–58.21 μg/ml). Strong binding affinities were recorded between fucosterol and Leishmania spp. trypanothione reductases. Conclusion: Fucosterol, which was abundant in S. vulgare, might be responsible for the antileishmanial activity.