Open AccessInductively coupled plasma mass spectrometry method for plasma and intracellular antimony quantification applied to pharmacokinetics of meglumine antimoniate
Author(s) -
Diana J. GarayBaquero,
David E. Rebellón-Sánchez,
Manuel Prieto,
Lina Giraldo-Parra,
Adriavas,
Sheryl Atkinson,
Stuart McDougall,
María Adelaida Gómez
Publication year - 2021
Publication title -
bioanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.566
H-Index - 58
eISSN - 1757-6199
pISSN - 1757-6180
DOI - 10.4155/bio-2021-0013
Subject(s) - meglumine antimoniate , cutaneous leishmaniasis , antimony , peripheral blood mononuclear cell , human plasma , medicine , chemistry , pharmacology , leishmaniasis , chromatography , immunology , in vitro , inorganic chemistry , biochemistry
Background: A high-throughput method using inductively coupled plasma mass spectrometry (ICP–MS) was developed and validated for the quantitative analysis of antimony in human plasma and peripheral blood mononuclear cells from patients with cutaneous leishmaniasis undergoing treatment with meglumine antimoniate. Materials & methods: Antimony was digested in clinical samples with 1% tetramethylammonium hydroxide/1% EDTA and indium was used as internal standard. Accuracy, precision and stability were evaluated. Conclusion: Taking the lower limit of quantitation to be the lowest validation concentration with precision and accuracy within 20%, the current assay was successfully validated from 25 to 10000 ng/ml for antimony in human plasma and peripheral blood mononuclear cells. This protocol will serve as a baseline for future analytical designs, aiming to provide a reference method to allow inter-study comparisons.