Open AccessExpression and prognostic significance of the DNA damage response pathway and autophagy markers in gastric cancer
Author(s) -
Xinbo Xu,
Nianshuang Li,
Huan Wang,
Yi Hu,
Xidong Wu,
Junbo Hong,
ghua Lü,
Chuan Xie
Publication year - 2021
Publication title -
neoplasma
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.628
H-Index - 50
eISSN - 1338-4317
pISSN - 0028-2685
DOI - 10.4149/neo_2021_210515n667
Subject(s) - autophagy , dna damage , cancer , immunohistochemistry , biology , cancer research , downregulation and upregulation , rad51 , dna , apoptosis , immunology , gene , genetics
Gastric cancer (GC) is a leading cause of mortality and morbidity worldwide. We assessed the expression patterns of DNA damage response (DDR)-related markers, including ATM, CHK2, p-p53 (S15), Rad51, and BRCA2 and autophagy-related proteins including p62 and Beclin-1 in 153 GC specimens using immunohistochemistry staining. GC tissues showed lower levels of ATM, CHK2, p-p53, BRCA2, and higher levels of Rad51 compared to adjacent normal tissues. The autophagy-related protein p62 was upregulated, whereas Beclin-1 was downregulated in human GC groups. Additionally, different statuses of DDR pathways and autophagy characterized by protein expression were associated with overall survival. Our results indicated that the impairment of DNA damage and autophagy may be implicated in gastric cancer progression and its clinical prognosis.