Open AccessmicroRNA-10a-5p overexpression suppresses malignancy of colon cancer by regulating human liver cancer fibroblasts
Author(s) -
Xuan Zheng,
Jingwu Li,
Yan-Kun Liu,
Yong Ma,
Jianhui Gan,
Song Han,
Jian Wang,
Zhao-yuan Wan,
Jun Zhang,
Yan Liu,
Yufeng Li,
Guangling Zhang
Publication year - 2021
Publication title -
neoplasma
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.628
H-Index - 50
eISSN - 1338-4317
pISSN - 0028-2685
DOI - 10.4149/neo_2021_210226n250
Subject(s) - metastasis , cancer associated fibroblasts , cancer research , liver cancer , cancer cell , stroma , colorectal cancer , microrna , cancer , tumor microenvironment , hepatic stellate cell , fibroblast , malignancy , chemistry , biology , pathology , medicine , cell culture , tumor cells , immunohistochemistry , hepatocellular carcinoma , biochemistry , genetics , gene
The crosstalk between tumor and stroma plays a critical role in cancer metastasis. However, the function of miR-10a-5p on liver fibroblasts in the metastatic microenvironment of colon cancer (CC) and the effect of activated fibroblasts on CC cells are still unclear. In our study, miR-10a-5p overexpression inhibited the proliferation, migration, and IL-6/IL-8 level of LX-2 cells and human liver cancer fibroblasts (HLCFs). Moreover, miR-10a-5p had lower expression in HLCFs than in human liver normal fibroblasts (HLNFs). The conditioned medium (CM) from LX-2 cells with miR-10a-5p overexpression or HLNFs could inhibit the invasion, migration, and stemness of CC SW480 cells, whereas HLCFs CM could promote these malignant phenotypes of SW480 cells. The present study illustrates the effect of miR-10a-5p on the liver fibroblasts and the altered liver fibroblasts in the microenvironment on CC cells induced by miR-10a-5p, which may aid the understanding of the mechanisms underlying CC liver metastasis.