Open AccessImpact of mesenchymal stem cells derived conditioned media on neural progenitor cells
Author(s) -
Filip Humeník,
Sonja Jego,
Lubica Hornakova,
Marcela Maloveská,
Alexandra Valenčáková-Agyagosová,
Zuzana Vikartovska,
Dagmar Mudroňová,
Nikola Hudáková,
Monika Žilková,
Dasa Cizkova Milan Cizek
Publication year - 2021
Publication title -
general physiology and biophysics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.376
H-Index - 39
eISSN - 1338-4325
pISSN - 0231-5882
DOI - 10.4149/gpb_2021026
Subject(s) - mesenchymal stem cell , progenitor cell , neurite , cd90 , microbiology and biotechnology , stem cell , neurotrophic factors , umbilical cord , neural stem cell , in vitro , progenitor , chemistry , biology , neuroscience , immunology , cd34 , receptor , biochemistry
Neurodegenerative diseases are common problem for companion animals. Due to the limited ability of injured axons to regenerate, innovative therapies combined with rehabilitation have been applied and evaluated. Among them, stem cells and their conditioned media implantation, which can ameliorate damaged tissue has been suggested as a promising treatment strategy. The main goal of our study was to characterize mesenchymal stem cells (MSC) derived from canine adipose tissue (AT-MSC) and umbilical cord (UC-MSC) and analyse effect of their conditioned media (CM) on neurite outgrowth of neural progenitor cells isolated from the brain cortex of neonatal rats. MSC from both sources showed high osteogenic and chondrogenic potential and expression of CD90 and CD29. Furthermore, both UC-MSCCM and AT-MSCCM stimulated neurite growth. Interestingly, this effect was more pronounced with UC-MSCCM when compared to AT-MSCCM in vitro, which may be related to the different content of neurotrophic factors included in the CM.