Open AccessInhibition of cytochrome P450 by proadifen diminishes the excitability of brain serotonin neurons in rats
Author(s) -
Daniil Grinchii,
Ruslan Paliokha,
Vadim Tseĭlikman,
Eliyahu Dremencov
Publication year - 2018
Publication title -
general physiology and biophysics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.376
H-Index - 39
eISSN - 1338-4325
pISSN - 0231-5882
DOI - 10.4149/gpb_2018040
Subject(s) - chloral hydrate , dorsal raphe nucleus , serotonin , microinjection , chemistry , pharmacology , raphe nuclei , saline , medicine , endocrinology , serotonergic , biology , receptor , biochemistry
The aim of this study was to investigate the effect an inhibitor of cytochrome-P450, proadifen hydrochloride (SKF525), on the excitability of serotonin neurons. Adult male Wistar rats were administered SKF525 forty-eight, twenty-four, and one hour before electrophysiological assessments. Control animals were injected saline. Rats were anesthetized with chloral hydrate and glass electrodes were stereotaxically inserted into the dorsal raphe nucleus (DRN). Serotonin neurons were identified and their firing activity was recorded. It was found that the SKF525 inhibits the excitability of 5-HT neurons. We suggest that corticosterone might play a key role in the SKF525-induced inhibition of 5-HT neurons.