Open AccessThe dosage-dependent prenatal caffeine exposure adversely affects levels of integrin αVβ3 and MMP-9 in a rat model of embryo implantation
Author(s) -
Filiz Yılmaz,
Işıl Tekmen
Publication year - 2020
Publication title -
bratislavské lekárske listy/bratislava medical journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.387
H-Index - 32
eISSN - 1336-0345
pISSN - 0006-9248
DOI - 10.4149/bll_2020_105
Subject(s) - embryo , matrix metalloproteinase , andrology , integrin , caffeine , chemistry , medicine , microbiology and biotechnology , endocrinology , biology , receptor
In the pregnancy period, it is recommended to limit the consumption of caffeine. However, the mechanisms of caffeine effect during pregnancy are not fully known. In our study, we aimed to investigate the effect of prenatal caffeine consumption on the embryonic implantation in rats as well as shed light on the relationship between the molecules and implantation stages.MATERIALS AND METHODS: Forty-five Wistar albino pregnant rats were randomly divided into 3 main groups, namely into control, low‑dose and high‑dose groups, representing the dose-dependent effects of caffeine. Each main group was divided into 3 subgroups, namely those to be sacrified on days 4 (pre-implantation), 5 (peri-implantation) and 6 (post-implantation) . Different doses of caffeine were given on consecutive days, starting from day 1 of pregnancy up to the day of euthanasia. The implantation sites were investigated with the use of hematoxylin et eosin, Masson trichrome and immunostaining of VEGF, MMP-9, integrin αVβ3, mucin-1 and HB-EGF.