Open AccessGeneration of a fluorescent oncoprotein in soluble form and its delivery into mammalian cells
Author(s) -
Fatemeh Motevalli,
A. Khodaei,
Masumeh Anvari,
Azam Bolhassani
Publication year - 2019
Publication title -
bratislavské lekárske listy/bratislava medical journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.387
H-Index - 32
eISSN - 1336-0345
pISSN - 0006-9248
DOI - 10.4149/bll_2019_017
Subject(s) - fusion protein , recombinant dna , inclusion bodies , protein tag , green fluorescent protein , flow cytometry , in vitro , histidine , affinity chromatography , escherichia coli , biochemistry , flag tag , microbiology and biotechnology , chemistry , myc tag , lac operon , urea , hek 293 cells , biology , amino acid , gene , enzyme
Protein fusion technology was widely used to improve expression, purification and solubility of the recombinant proteins expressed in E. coli for in vitro/in vivo delivery.