Generation of a fluorescent oncoprotein in soluble form and its delivery into mammalian cells | Zendy

F Motevalli | Zendy; A. Khodaei | Zendy; Masoumeh Anvari | Zendy; Azam Bolhassani | Zendy

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Generation of a fluorescent oncoprotein in soluble form and its delivery into mammalian cells

Author(s) -

F Motevalli,

A. Khodaei,

Masoumeh Anvari,

Azam Bolhassani

Publication year - 2019

Publication title -

bratislavské lekárske listy/bratislava medical journal

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.387

H-Index - 32

eISSN - 1336-0345

pISSN - 0006-9248

DOI - 10.4149/bll_2019_017

Subject(s) - fusion protein , recombinant dna , inclusion bodies , protein tag , green fluorescent protein , flow cytometry , in vitro , histidine , affinity chromatography , escherichia coli , biochemistry , flag tag , microbiology and biotechnology , chemistry , myc tag , lac operon , urea , hek 293 cells , biology , amino acid , gene , enzyme

Protein fusion technology was widely used to improve expression, purification and solubility of the recombinant proteins expressed in E. coli for in vitro/in vivo delivery.

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