Open AccessNeuron-specific enolase in the intestinal wall in Crohn´s disease
Author(s) -
P Busikova-Malenovska,
Dianne Danis,
M Bencat,
Paulína Gálfiová,
Martin Kopáni,
V Labajova,
O. El Hassoun,
J Porubský,
J Galatova
Publication year - 2014
Publication title -
bratislavské lekárske listy/bratislava medical journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.387
H-Index - 32
eISSN - 1336-0345
pISSN - 0006-9248
DOI - 10.4149/bll_2014_075
Subject(s) - enolase , clone (java method) , monoclonal antibody , crohn's disease , pathology , epitope , biopsy , monoclonal , microbiology and biotechnology , disease , medicine , biology , antibody , chemistry , immunohistochemistry , immunology , biochemistry , gene
The authors described the localization of neuron-specific enolase in the intestinal wall in Crohn´s disease. We have used samples obtained by biopsy from the colon lining of five people affected by Crohn's disease for our examination. We have processed samples using the formol paraffin technique. From paraffin blocks, we have prepared histological sections approximately 5 μm thick. For immunohistochemic examinations, we have revitalised the sections by acquiring the heat-induced epitope. We detected NSE by monoclonal mouse antibodies against human neuron-specific enolase, clone BBS/NC/VI-H14 (DakoCytomation, Denmark) (Fig. 4, Ref. 7).