Open AccessComplete genome sequence and genetic organization of a Garlic virus D infecting garlic (Allium sativum) from northern India
Author(s) -
Jitender Singh,
Thao Truong,
Dong-Jun An,
Malyaj R Prajapati,
Aakansha Manav,
Nguyễn Bảo Quốc,
Pankaj Kumar,
Ravindra Kumar,
V. K. Baranwal
Publication year - 2020
Publication title -
acta virologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.412
H-Index - 33
eISSN - 1336-2305
pISSN - 0001-723X
DOI - 10.4149/av_2020_405
Subject(s) - allium sativum , biology , whole genome sequencing , genome , allium , botany , virology , genetics , gene
The present paper describes first full genome sequence of the Garlic virus D (GarV-D) from northern India with a genome size of 8425 bp long ssRNA. The infected leaves and bulbs of garlic variety Yamuna Safed (G-282) plants suspected for GarV-D infection were collected with the aim to identify contagion virus during March, 2018. The total RNA was extracted from the pooled garlic plants using TRIzol reagent and sequenced using an Illumina HiSeq 2000 platform. BLASTn search in the NCBI database identified contagion as GarV-D (MK518067). It shared 83.63-85.83% nucleotide sequence identities with other (GarV-D) isolates from Argentina (KF550407, KF555653, KR819505) and 83.15% with isolates from China (MF795136, MF363012). Keywords: Allium sativum; Allexivirus; Garlic virus D; India.