Open AccessE. coli expression and immunological assessment of expressed recombinant Newcastle disease virus hemagglutinin-neuraminidase protein in chickens
Author(s) -
Nigar S. Shahid,
Arundhati Rao,
Abdul Ahad,
Aytül Gül,
Ayesha Latif,
Saira Azam,
Muhammad Akbar Shahid,
Sameera Akhtar,
Ahmed Ali Shahid,
Tayyab Husnain
Publication year - 2020
Publication title -
acta virologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.412
H-Index - 33
eISSN - 1336-2305
pISSN - 0001-723X
DOI - 10.4149/av_2020_310
Subject(s) - virology , neuraminidase , newcastle disease , hemagglutinin (influenza) , biology , recombinant dna , virus , microbiology and biotechnology , gene , biochemistry
Every year, the poultry industry experiences significant economic losses due to epidemics of Newcastle disease virus (NDV). Developing new vaccines by identifying and using the immunogenic hemagglutinin-neuraminidase (HN) protein can protect the poultry industry. In the present study, the full-length HN protein was expressed in Escherichia coli (E. coli) BL21 (DE3) cells, purified via affinity chromatography and detected via western blot analysis using His-specific antibodies. The purified HN protein was further evaluated in chickens to study the immune response against NDV. The successful production of HN-specific IgY proved the activity of the purified HN protein. IgY was present in the serum of immunized chickens. However, the immune response was higher in chickens immunized with purified HN protein along with complete and incomplete adjuvants than in chickens immunized with only the HN protein. Keywords: protein; Newcastle disease virus; poultry; infectious diseases; vaccines.