Open AccessMicroRNA-185 reduces the expression of hepatitis B virus surface antigen by targeting PRKCH in HepG2 2.2.15 cells
Author(s) -
Ronghua Zhang,
Zhangxinyi Liu,
Xiaoli Hou,
Meimei Wang,
Yong Xiong,
Hongjian Lu,
Yitong Wang,
Jinghui Su,
Yutan Liu,
Guangling Zhang,
Yakun Liu
Publication year - 2020
Publication title -
acta virologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.412
H-Index - 33
eISSN - 1336-2305
pISSN - 0001-723X
DOI - 10.4149/av_2020_304
Subject(s) - virology , antigen , hepatitis b virus , virus , microrna , biology , microbiology and biotechnology , immunology , gene , genetics
MicroRNAs (miRNAs) are single-stranded noncoding RNAs with 18 to 25 nucleotides and play critical roles in a wide spectrum of biological processes. We repored that miR-185 inhibited hepatitis B surface antigen (HBsAg) expression and hepatitis B virus (HBV) replication without affecting the proliferation of HepG2 2.2.15 cells, compared with the controls. We identified that protein kinase C eta (PRKCH) is a direct target gene of miR-185 that affects HBV replication and protein expression and that the miR-185 may suppress HBV replication. Our results provide more information for gene therapy in HBV infection. Keywords: miR-185; HBV; HBV surface antigen; viral replication; PRKCH.