Open AccessKaempferol inhibited bovine herpesvirus 1 replication and LPS-induced inflammatory response
Author(s) -
Linnan Zhu,
P Wang,
Wenbing Yuan,
Guangfa Zhu
Publication year - 2018
Publication title -
acta virologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.412
H-Index - 33
eISSN - 1336-2305
pISSN - 0001-723X
DOI - 10.4149/av_2018_206
Subject(s) - kaempferol , u937 cell , tumor necrosis factor alpha , in vitro , viral replication , bovine herpesvirus 1 , biology , virology , virus , quercetin , herpesviridae , immunology , biochemistry , viral disease , antioxidant
Plant-derived flavonoids contain large amount of compounds with pharmacological effects. In this study, we showed the compound Kaempferol to have robust antiviral activity against bovine herpesvirus 1 (BoHV-1) replication in vitro. Kaempferol at a concentration of 100 μmol/l completely inhibited viral replication in MDBK cells. It mainly affects the viral replication at the post-entry stages. The inhibition of Akt signaling is a potential mechanism underlying the antiviral effect of Kaempferol. In addition, at a concentration of 25 and 50 μmol/l Kaempferol could significantly reduce the expression of inflammatory mediators such as tumor necrosis factor alpha (TNF-α), interleukin-8 (IL-8) and macrophage inflammatory protein 1 alpha (MIP-1α) in human promonocytic U937 cells-derived macrophages (dU937) in response to lipopolysaccharides (LPS) stimulation. Overall, our results indicated that Kaempferol provides a potent protection against BoHV-1 infection and LPS-induced inflammatory response.