Open AccessGenetic diversity of the nucleocapsid protein gene of hippeastrum chlorotic ringspot virus from Hymenocallis littoralis in southern China
Author(s) -
Q. LI,
Yifan Xu,
Miao Zhu,
Yin Ding,
Jiafen Hu,
Y. LI,
Y. LIU
Publication year - 2017
Publication title -
acta virologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.412
H-Index - 33
eISSN - 1336-2305
pISSN - 0001-723X
DOI - 10.4149/av_2017_01_116
Subject(s) - biology , virology , giant virus , gene , polyclonal antibodies , virus , plant virus , antibody , genetics , genome
Mimivirus was the first discovered amoebal giant virus. The Mimivirus virions are covered by a dense layer of approximately 130 nm-long fibers, the length and shape of which diverge from those of other viruses. Here, we aimed at expressing the L725 protein to further confirm and study its role as a fiber-associated protein. We report Escherichia coli expression of the L725 protein, which is encoded by a Mimivirus ORFan, was previously identified by proteomics in purified viral fibers and demonstrated to be a fiber-associated protein by RNA-silencing experiments. The expressed protein was recognized by anti-Mimivirus fiber or anti-Mimivirus L725 polyclonal antibodies. This study is the only expression, to our knowledge, of a product from a Mimiviral ORFan gene.