Open AccessPreparation and identification of an antiserum against recombinant UL31 protein of pseudorabies virus
Author(s) -
Mingsheng Cai,
Shibo Jiang,
Chengjun Mo,
J L Wang,
Junfeng Huang,
Zhenghai Zeng,
X W Li,
Yujie Yang,
C K Chen,
Wen Cui,
M L Li
Publication year - 2015
Publication title -
acta virologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.412
H-Index - 33
eISSN - 1336-2305
pISSN - 0001-723X
DOI - 10.4149/av_2015_03_295
Subject(s) - antiserum , pseudorabies , immunofluorescence , virology , biology , recombinant dna , virus , microbiology and biotechnology , antigen , antibody , immunology , genetics , gene
Pseudorabies virus (PRV) early protein UL31 is a homologue of herpes simplex virus 1 (HSV-1) UL31, which is a multifunctional protein important for HSV-1 infection. However, the precise roles of PRV UL31 in virus life cycle are still poorly understood. A relatively crucial tool for uncovering the function of UL31 is an antiserum that specifically detects UL31 in the PRV-infected cells. For this purpose, a recombinant UL31 protein consisting of N-terminal 27 aa of UL31 fused to EYFP and His-tag was expressed, purified and used for the preparation of antiserum in BALB/c mice. Our results show that Western blot analysis and immunofluorescence assay showed that this antiserum could specifically detect the purified recombinant UL31 as well as full-length UL31 in the PRV infected cells. These results demonstrate that the prepared antiserum could serve as a valuable tool for further studies of UL31 functions in PRV infection.