Open AccessMolecular and genomic features ofMycobacterium bovisstrain 1595 isolated from Korean cattle
Author(s) -
Na-Rae Kim,
Yunho Jang,
Jin Kyoung Kim,
Soyoon Ryoo,
Kui-Kam Kwon,
Miso Kim,
Shin Seok Kang,
Hyeon Seop Byeon,
Hee Soo Lee,
Young Hee Lim,
JaeMyung Kim
Publication year - 2017
Publication title -
journal of veterinary science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 47
eISSN - 1976-555X
pISSN - 1229-845X
DOI - 10.4142/jvs.2017.18.s1.333
Subject(s) - mycobacterium bovis , genome , biology , phylogenetic tree , genetics , whole genome sequencing , strain (injury) , tandem repeat , sequence analysis , variable number tandem repeat , gene , genotype , mycobacterium tuberculosis , tuberculosis , medicine , pathology , anatomy
The aim of this study was to investigate the molecular characteristics and to conduct a comparative genomic analysis of Mycobacterium (M.) bovis strain 1595 isolated from a native Korean cow. Molecular typing showed that M. bovis 1595 has spoligotype SB0140 with mycobacterial interspersed repetitive units-variable number of tandem repeats typing of 4-2-5-3-2-7-5-5-4-3-4-3-4-3, representing the most common type of M. bovis in Korea. The complete genome sequence of strain 1595 was determined by single-molecule real-time technology, which showed a genome of 4351712 bp in size with a 65.64% G + C content and 4358 protein-coding genes. Comparative genomic analysis with the genomes of Mycobacterium tuberculosis complex strains revealed that all genomes are similar in size and G + C content. Phylogenetic analysis revealed all strains were within a 0.1% average nucleotide identity value, and MUMmer analysis illustrated that all genomes showed positive collinearity with strain 1595. A sequence comparison based on BLASTP analysis showed that M. bovis AF2122/97 was the strain with the greatest number of completely matched proteins to M. bovis 1595. This genome sequence analysis will serve as a valuable reference for improving understanding of the virulence and epidemiologic traits among M. bovis isolates in Korea.