Open AccessMicrotubule distribution in somatic cell nuclear transfer bovine embryos following control of nuclear remodeling type
Author(s) -
Dae Jin Kwon,
Yu Mi Lee,
In-Sul Hwang,
Choon Keun Park,
BooKeun Yang,
Hee-Tae Cheong
Publication year - 2010
Publication title -
journal of veterinary science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 47
eISSN - 1976-555X
pISSN - 1229-845X
DOI - 10.4142/jvs.2010.11.2.93
Subject(s) - somatic cell nuclear transfer , premature chromosome condensation , microtubule , biology , embryo , somatic cell , mitosis , microbiology and biotechnology , andrology , genetics , cell , embryogenesis , cell cycle , blastocyst , medicine , gene
This study was conducted to evaluate the microtubule distribution following control of nuclear remodeling by treatment of bovine somatic cell nuclear transfer (SCNT) embryos with caffeine or roscovitine. Bovine somatic cells were fused to enucleated oocytes treated with either 5 mM caffeine or 150 microM roscovitine to control the type of nuclear remodeling. The proportion of embryos that underwent premature chromosome condensation (PCC) was increased by caffeine treatment but was reduced by roscovitine treatment (p < 0.05). The microtubule organization was examined by immunostaining beta- and gamma-tubulins at 15 min, 3 h, and 20 h of fusion using laser scanning confocal microscopy. The gamma-tubulin foci inherited from the donor centrosome were observed in most of the SCNT embryos at 15 min of fusion (91.3%) and most of them did not disappear until 3 h after fusion, regardless of treatment (82.9-87.2%). A significantly high proportion of embryos showing an abnormal chromosome or microtubule distribution was observed in the roscovitinetreated group (40.0%, p < 0.05) compared to the caffeinetreated group (22.1%). In conclusion, PCC is a favorable condition for the normal organization of microtubules, and inhibition of PCC can cause abnormal mitotic division of bovine SCNT embryos by causing microtubule dysfunction.