Open AccessDetermination of ethinyl estradiol and levonorgestrel in human plasma with prednisone as internal standard using ultra-performance liquid chromatography–tandem mass spectrometry
Author(s) -
Yahdiana Harahap,
Devina Devina,
Harmita Harmita
Publication year - 2019
Publication title -
journal of pharmacy and bioallied sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.268
H-Index - 36
eISSN - 0976-4879
pISSN - 0975-7406
DOI - 10.4103/jpbs.jpbs_68_19
Subject(s) - chromatography , chemistry , levonorgestrel , formic acid , electrospray ionization , protein precipitation , liquid chromatography–mass spectrometry , tandem mass spectrometry , high performance liquid chromatography , mass spectrometry , solid phase extraction , population , medicine , environmental health , family planning , research methodology
Ethinyl estradiol and levonorgestrel as a combination of oral contraceptive drugs have very low dosage levels; hence, a highly sensitive and selective method of using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) is needed.