Open AccessEvaluation of the antidiabetic potential of an isolated hydroalcoholic fraction from the fruit of withania coagulans
Author(s) -
Md. Waris,
Naiyer Shahzad,
Saeed S. AlGhamdi,
Showkat R. Mir,
Tanuja
Publication year - 2021
Publication title -
journal of pharmacy and bioallied sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.268
H-Index - 36
eISSN - 0976-4879
pISSN - 0975-7406
DOI - 10.4103/jpbs.jpbs_423_20
Subject(s) - phytochemical , chemistry , traditional medicine , chromatography , ethyl acetate , starch , carbohydrate , food science , medicine , biochemistry
The hydro-alcoholic extract of Withania coagulans fruits was investigated for preliminary phytochemical screening and characterized by high-performance thin-layer chromatography. Column chromatography of the hydro-alcoholic extract of W. coagulans eluted with four different combinations of ethyl acetate and methanol yielded four fractions (WCF 01 , WCF 02 , WCF 03, and WCF 04 ). One of these fractions, WCF 02 , significantly ( P < 0.05) inhibited in vitro α-amylase and α-glucosidase activity with IC 50 values of 104.71 μg/mL and 70.79 μg/mL, respectively. WCF 02 further reduced blood glucose levels in comparison to control in the starch tolerance test. The extract showed a relative dose-dependent effect. It was observed that none of the extracts could delay the peak blood glucose that was achieved after 60 min of carbohydrate challenge, but these blunted the glycemic peak.