Open AccessEx vivo culture of oral keratinocytes using direct explant cell culture technique
Author(s) -
HR Shwetha,
Vijayalakshmi S Kotrashetti,
Nishith Babu,
Vijay Kumbar,
Kishore Bhat,
Roopa Reddy
Publication year - 2019
Publication title -
journal of oral and maxillofacial pathology/journal of oral and maxillofacial pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.455
H-Index - 25
eISSN - 1998-393X
pISSN - 0973-029X
DOI - 10.4103/jomfp.jomfp_105_19
Subject(s) - explant culture , ex vivo , cell culture , in vivo , microbiology and biotechnology , in vitro , biology , genetics
Culture of cells and tissues is a standard research method practiced in many laboratories. In most of the cases, these cultures are being used as substrates for cell products or as investigative tools for delving the mechanism of gene expression, cell proliferation and transformation. Primary monolayer cell culture has been beneficial to study the general biology of both oral and skin keratinocytes. There are two different techniques of primary cell cultures followed, which include direct explant and enzymatic techniques.