Open AccessDevelopment of a dry-reagent mix-based polymerase chain reaction as a novel tool for the identification of Acinetobacter species and its comparison with conventional polymerase chain reaction
Author(s) -
Raghavendra Kulkarni,
Mukti Nath Mishra,
Jeevanandam Mohanraj,
Arun Chandrasekhar,
G S Ajantha,
Sheetal Kulkani,
Shama Bhat
Publication year - 2018
Publication title -
journal of laboratory physicians
Language(s) - English
Resource type - Journals
eISSN - 0974-7826
pISSN - 0974-2727
DOI - 10.4103/jlp.jlp_74_17
Subject(s) - reagent , acinetobacter , acinetobacter baumannii , microbiology and biotechnology , polymerase chain reaction , biology , chromatography , bacteria , chemistry , antibiotics , biochemistry , gene , organic chemistry , genetics , pseudomonas aeruginosa
Nosocomial infections are often caused by multidrug-resistant bacteria and the incidence is increasing. Acinetobacter , a Gram-negative bacillus, is commonly associated with the use of intravascular catheterization and airway intubation. Polymerase chain reaction (PCR) for identification of Acinetobacter baumannii from samples has been standardized that use conventional wet-reagent mix. We have designed and optimized a dry-reagent mix for identification of Acinetobacter species by PCR. The dry-reagent mix can be stored at room temperature, has less chances of contamination, and thus can be used at point-of-care diagnosis.