Open AccessTechnetium-99m-labeled genistein as a potential radical scavenging agent
Author(s) -
Danni Ramdhani,
Maula Eka Sriyani,
Flamboyan Ayu
Publication year - 2019
Publication title -
journal of advanced pharmaceutical technology and research
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.325
H-Index - 33
eISSN - 2231-4040
pISSN - 0976-2094
DOI - 10.4103/japtr.japtr_27_19
Subject(s) - genistein , chemistry , technetium , incubation , chromatography , radiochemistry , technetium 99m , nuclear chemistry , biochemistry , nuclear medicine , scintigraphy , medicine
The purpose of this study was to determine the optimum conditions for labeling genistein compounds with technetium-99m ( 99m Tc) radionuclides and the percentage of purity obtained in accordance with the requirements of the United State Pharmacopeia. The method used is optimization of several parameters including pH, SnCl 2 .2H 2 O as reducing agents, genistein concentration, and incubation time. The results showed that the optimum conditions for labeling 99m Tc-Genistein were obtained under conditions of pH 8, the amount of SnCl 2 .2H 2 O reducing agents was 30 μg, 0.5 mg genistein, and in 10 min. The optimization of this condition resulted in radiochemical purity in the labeling of 99m Tc-Genistein compounds at 95.43% ± 0.85%. The radiochemical purity of the labeling of 99m Tc-Genistein compounds has met the requirements of the United State Pharmacopeia as a compound marked for diagnosis of more than 90%.