Open AccessIn vitro anticancer activity Annona squamosa extract nanoparticle on WiDr cells
Author(s) -
Amaq Fadholly,
Annise Proboningrat,
Regina Purnama Dewi Iskandar,
Fedik Abdul Rantam,
Sri Agus Sudjarwo
Publication year - 2019
Publication title -
journal of advanced pharmaceutical technology and research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.325
H-Index - 33
eISSN - 2231-4040
pISSN - 0976-2094
DOI - 10.4103/japtr.japtr_10_19
Subject(s) - annona squamosa , dimethyl sulfoxide , in vivo , ic50 , apoptosis , bromide , cytotoxicity , in vitro , cell cycle , nanoparticle , chemistry , nuclear chemistry , biochemistry , pharmacology , nanotechnology , biology , materials science , botany , organic chemistry , microbiology and biotechnology
This study aimed to prepare Annona squamosa leaf extract-loaded chitosan nanoparticles (nano-ASLE) against human colon cancer (WiDr) cells. Nano-ASLE was made with ionic gelation method. Four concentrations of the nano-ASLE (50, 100, 200, and 400 μg/mL) in dimethyl sulfoxide were prepared on WiDr cells to determine the IC 50 value using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Then, it was divided into three groups of concentration of IC 50 , 2IC 50 , and 4IC 50 and continued with analysis of caspase-3 expression and cell cycle arrest. The results of particles size were obtained 535.1 nm and showed potent cytotoxicity with IC 50 292.39 μg/mL. The expression of caspase-3 increased significantly and caused cell cycle arrest at the G2/M phase and induced apoptosis on WiDr cells. Further studies are needed to obtain the loading efficiency, release of drug concentration, and in vivo study of nano-ASLE to suppress WiDr cells.