Open AccessMultiplex nested polymerase chain reaction targeting multiple genes for the detection of Neisseria gonorrhoeae and Chlamydia trachomatis in genitourinary specimens
Author(s) -
Ketan Priyadarshi,
Pradyot Prakash,
Anjali Rani,
Satyendra Kumar Singh
Publication year - 2019
Publication title -
indian journal of sexually transmitted diseases and aids
Language(s) - English
Resource type - Journals
eISSN - 2589-0565
pISSN - 2589-0557
DOI - 10.4103/ijstd.ijstd_73_18
Subject(s) - neisseria gonorrhoeae , chlamydia trachomatis , nested polymerase chain reaction , multiplex polymerase chain reaction , virology , multiplex , amplicon , polymerase chain reaction , medicine , microbiology and biotechnology , coinfection , sexually transmitted disease , biology , gene , syphilis , genetics , virus , human immunodeficiency virus (hiv)
The objective of this study was to design and evaluate a novel multiplex nested polymerase chain reaction (PCR) protocol for simultaneous detection of Neisseria gonorrhoeae and Chlamydia trachomatis in genitourinary specimens obtained from symptomatic patients clinically suspected of sexually transmitted infections (STIs), targeting two different genes each for these pathogens.