Open AccessEvaluation of multiplex real-time polymerase chain reaction for the detection of herpes simplex virus-1 and 2 and varicella-zoster virus in corneal cells from normal subjects and patients with keratitis in India
Author(s) -
Sai Jeevana Madhuri Guda,
Bhavani Sontam,
Bhupesh Bagga,
Konduri Ranjith,
Savitri Sharma,
Joveeta Joseph
Publication year - 2019
Publication title -
indian journal of ophthalmology/indian journal of ophthalmology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.542
H-Index - 51
eISSN - 1998-3689
pISSN - 0301-4738
DOI - 10.4103/ijo.ijo_1700_18
Subject(s) - herpes simplex virus , medicine , keratitis , varicella zoster virus , virus , real time polymerase chain reaction , virology , multiplex , cornea , polymerase chain reaction , herpesviridae , photorefractive keratectomy , multiplex polymerase chain reaction , immunofluorescence , immunology , viral disease , ophthalmology , antibody , biology , gene , bioinformatics , biochemistry
To determine the presence of herpes simplex virus and varicella zoster virus (HSV 1 and 2, VZV) in the cornea of normal subjects by multiplex real time quantitative (qPCR) assay and evaluate its utility in the diagnosis of viral keratitis.