Open AccessDevelopment of a RT-LAMP assay for detection of SARS-CoV-2
Author(s) -
Shyam Sundar Nandi,
Upendra P. Lambe,
Sonali Sawant,
Trupti Gohil,
Jagadish M. Deshpande
Publication year - 2022
Publication title -
indian journal of medical research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.578
H-Index - 87
ISSN - 0971-5916
DOI - 10.4103/ijmr.ijmr_713_21
Subject(s) - loop mediated isothermal amplification , reverse transcription polymerase chain reaction , reverse transcription loop mediated isothermal amplification , real time polymerase chain reaction , virology , reverse transcriptase , covid-19 , polymerase chain reaction , biology , microbiology and biotechnology , medicine , gene , dna , messenger rna , pathology , genetics , disease , infectious disease (medical specialty)
The pandemic of SARS-COV-2 began in Wuhan, China in December 2019 and has caused more than 101 million cases worldwide. Diagnostic technologies possessing sensitivity and specificity equivalent to real-time reverse-transcriptase polymerase chain reaction (rRT-PCR) assays are needed to ramp up testing capacity in most countries. Newer platforms need to be technically less demanding, require minimum equipment and reduce turn-around time for reporting results. The objective of this study was to exploit loop-mediated isothermal amplification (LAMP) for the detection of SARS-CoV-2 and evaluate its performance by comparison with rRT-PCR.