Open AccessGenotype analysis of ofloxacin-resistant multidrug-resistant Mycobacterium tuberculosis isolates in a multicentered study from India
Author(s) -
Anamika Gupta,
Pallavi Sinha,
Sunita Rathod,
Siva Kumar Shanmugam,
Kangjam Rekha Devi,
Shampa Anupurba,
Vijay Nema
Publication year - 2020
Publication title -
indian journal of medical research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.578
H-Index - 87
ISSN - 0971-5916
DOI - 10.4103/ijmr.ijmr_493_18
Subject(s) - ofloxacin , genotype , biology , mycobacterium tuberculosis , tuberculosis , polymerase chain reaction , drug resistance , multiple drug resistance , microbiology and biotechnology , dna gyrase , gene , veterinary medicine , antibiotics , virology , genetics , medicine , ciprofloxacin , escherichia coli , pathology
Drug resistance surveillance offers useful information on trends of drug resistance and the efficacy of control measures. Studies and reports of drug-resistant mutations and phenotypic assays thus become important. This study was conducted to investigate the molecular characteristics of ofloxacin (OFX)-resistant, multidrug-resistant tuberculosis (MDR-TB) isolates from different geographical regions of India and their association with strains of different genotypes. Further, the nitrate reductase assay (NRA) was tested against Mycobacteria Growth Indicator Tube(MGIT) for the determination of OFX resistance as an alternative and cost-effective method.