Open AccessMolecular genotyping of clinically important blood group antigens in patients with thalassaemia
Author(s) -
Shrikant Kulkarni,
Bhavika Choudhary,
Harita Gogri,
Shashikant Patil,
Mamta Manglani,
Rachana Sharma,
Manisha Madkaikar
Publication year - 2018
Publication title -
indian journal of medical research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.578
H-Index - 87
ISSN - 0971-5916
DOI - 10.4103/ijmr.ijmr_455_17
Subject(s) - genotyping , antigen , immunology , serology , medicine , hemagglutination , blood transfusion , rh blood group system , genotype , virology , biology , antibody , genetics , gene
In multitransfused thalassaemic patients, haemagglutination fails to phenotype the patient's blood group antigens due to the presence of donor-derived erythrocytes. DNA-based methods can overcome the limitations of haemagglutination and can be used to determine the correct antigen profile of these patients. This will facilitate the procurement of antigen-matched blood for transfusion to multitransfused patients. Thus, the aim of this study was to compare the serological phenotyping of common and clinically important antigens of Rh, Duffy, Kell, Kidd and MNS blood group systems with molecular genotyping amongst multitransfused thalassaemic patients.