Open AccessFeeder & basic fibroblast growth factor-free culture of human embryonic stem cells: Role of conditioned medium from immortalized human feeders
Author(s) -
Pooja Teotia,
Shilpa Sharma,
Balram Airan,
Sujata Mohanty
Publication year - 2016
Publication title -
indian journal of medical research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.578
H-Index - 87
ISSN - 0971-5916
DOI - 10.4103/ijmr.ijmr_424_15
Subject(s) - basic fibroblast growth factor , embryonic stem cell , cell culture , microbiology and biotechnology , immortalised cell line , biology , embryoid body , fibroblast growth factor , kosr , fibroblast , growth factor , immunology , adult stem cell , genetics , receptor , gene
Human embryonic stem cell (hESC) lines are commonly maintained on inactivated feeder cells, in the medium supplemented with basic fibroblast growth factor (bFGF). However, limited availability of feeder cells in culture, and the high cost of growth factors limit their use in scalable expansion of hESC cultures for clinical application. Here, we describe an efficient and cost-effective feeder and bFGF-free culture of hESCs using conditioned medium (CM) from immortalized feeder cells.