Open AccessRapid detection of drug-resistant Mycobacterium tuberculosis directly from clinical specimens using allele-specific polymerase chain reaction assay
Author(s) -
Pallavi Sinha,
Tuhina Banerjee,
Govind Srivastava,
Shampa Anupurba
Publication year - 2019
Publication title -
indian journal of medical research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.578
H-Index - 87
ISSN - 0971-5916
DOI - 10.4103/ijmr.ijmr_374_18
Subject(s) - rpob , variants of pcr , polymerase chain reaction , mycobacterium tuberculosis , biology , isoniazid , rifampicin , inha , tuberculosis , nested polymerase chain reaction , genotype , allele , drug resistance , microbiology and biotechnology , virology , medicine , antibiotics , genetics , gene , pathology
Rapid detection of drug resistance in Mycobacterium tuberculosis (MTB) is essential for the efficient control of tuberculosis. Hence, in this study a nested-allele-specific (NAS) PCR, nested multiple allele-specific PCR (NMAS-PCR) and multiple allele-specific (MAS) PCR assays were evaluated that enabled detection of the most common mutations responsible for isoniazid (INH) and rifampicin (RIF) resistance in MTB isolates directly from clinical specimens.