Open AccessMulticentric validation of indigenous molecular test Truenat™ MTB for detection of Mycobacterium tuberculosis in sputum samples from presumptive pulmonary tuberculosis patients in comparison with reference standards
Author(s) -
N S Gomathi,
Manjula Singh,
Urvashi Singh,
V P Myneedu,
D. S. Chauhan,
Rohit Sarin,
Anant Mohan,
Anuj Bhatnagar,
Jiten Singh Khangembam,
T Kannan,
Mohan Rao,
Jyoti Logani,
Bindu Dey,
Raman Gangakhedkar,
Soumya Swaminathan,
Srikanth Tripathy
Publication year - 2020
Publication title -
indian journal of medical research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.578
H-Index - 87
ISSN - 0971-5916
DOI - 10.4103/ijmr.ijmr_2539_19
Subject(s) - sputum , medicine , tuberculosis , mycobacterium tuberculosis , nucleic acid amplification tests , gold standard (test) , virology , pathology , chlamydia trachomatis
: Early case detection is essential to interrupt transmission and to prevent further spread of tuberculosis (TB) in high endemic settings. Nucleic acid amplification tests (NAATs) with visual read-outs are ideal as point-of-care tests. Truenat™ MTB is an indigenous chip-based NAAT for detection of Mycobacterium tuberculosis, which involves extraction of DNA and real-time polymerase chain reaction (PCR) using portable, automated, battery-operated instruments. The current multicentric study was aimed to evaluate Truenat for detection of MTB in sputum samples obtained from patients with presumptive pulmonary TB with reference to culture as gold standard and Xpert as a comparator.