Open AccessDistribution of carbapenemase genes in clinical isolates of Acinetobacter baumannii & a comparison of MALDI-TOF mass spectrometry-based detection of carbapenemase production with other phenotypic methods
Author(s) -
Megha Sharma,
Lipika Singhal,
Vikas Gautam,
Pallab Ray
Publication year - 2020
Publication title -
indian journal of medical research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.578
H-Index - 87
ISSN - 0971-5916
DOI - 10.4103/ijmr.ijmr_1383_18
Subject(s) - acinetobacter baumannii , microbiology and biotechnology , carbapenem , multiplex , imipenem , multiplex polymerase chain reaction , acinetobacter , biology , antimicrobial , gene , pseudomonas aeruginosa , bacteria , antibiotics , antibiotic resistance , bioinformatics , polymerase chain reaction , genetics
Carbapenemase-producing Acinetobacter baumannii (CRAB) poses a continuous threat to the current antimicrobial era with its alarming spread in critical care settings. The present study was conducted to evaluate the diagnostic potential of phenotypic methods for carbapenemase [carbapenem-hydrolyzing class D β-lactamases (CHDLs) and metallo-β-lactamases (MBLs)] production, by comparing with molecular detection of genes.