Open AccessDevelopment of immunodetection system for botulinum neurotoxin serotype E
Author(s) -
R Sarita,
S. Ponmariappan,
Ashu Sharma,
Dev Vrat Kamboj,
Ashok Kumar Jain
Publication year - 2018
Publication title -
indian journal of medical research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.578
H-Index - 87
ISSN - 0971-5916
DOI - 10.4103/ijmr.ijmr_1375_16
Subject(s) - polyclonal antibodies , microbiology and biotechnology , clostridium botulinum , recombinant dna , botulism , escherichia coli , serotype , affinity chromatography , gel electrophoresis , biology , chemistry , antibody , toxin , enzyme , biochemistry , gene , immunology
Botulism, a potentially fatal paralytic illness, is caused by the botulinum neurotoxins (BoNTs) secreted by Clostridium botulinum. It is an obligate anaerobic, Gram-positive, spore-forming bacterium. BoNTs are classified into seven serotypes based on the serological properties. Among these seven serotypes, A, B, E and, rarely, F are responsible for human botulism. The present study was undertaken to develop an enzyme-linked immunosorbent assay (ELISA)-based detection system for the detection of BoNT/E.