Open AccessComparison of Two Real-time Polymerase Chain Reaction Assays for the Detection of Severe Acute Respiratory Syndrome-CoV-2 from Combined Nasopharyngeal-Throat Swabs
Author(s) -
Oves Siddiqui,
Vikas Manchanda,
Abhishek Yadav,
Tanu Sagar,
Sanchita Tuteja,
Nazia Nagi,
Sonal Saxena
Publication year - 2020
Publication title -
indian journal of medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1998-3646
pISSN - 0255-0857
DOI - 10.4103/ijmm.ijmm_20_279
Subject(s) - concordance , gold standard (test) , medicine , polymerase chain reaction , real time polymerase chain reaction , throat , covid-19 , respiratory system , virology , context (archaeology) , kappa , biology , surgery , gene , disease , biochemistry , infectious disease (medical specialty) , paleontology , linguistics , philosophy
In the absence of effective treatment or vaccine, the current strategy for the prevention of further transmission of severe acute respiratory syndrome (SARS) CoV-2 (COVID-19) infection is early diagnosis and isolation of cases. The diagnosis of SARS-CoV-2 is done by detecting viral RNA in the nasopharyngeal and throat swabs by real-time polymerase chain reaction (PCR). Many commercial assays are now available for performing the PCR assay.