Open AccessCharacterization of the biological effect of BiodentineTM on primary dental pulp stem cells
Author(s) -
Nadine Hasweh,
Abdalla Awidi,
Lamis D. Rajab,
Ahmad Hiyasat,
Hanan Jafar,
Najmul Islam,
Maram Hasan,
Duaa Abuarqoub
Publication year - 2018
Publication title -
indian journal of dental research/indian journal of dental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.277
H-Index - 43
eISSN - 1998-3603
pISSN - 0970-9290
DOI - 10.4103/ijdr.ijdr_28_18
Subject(s) - mtt assay , adhesion , dental pulp stem cells , andrology , cell growth , chemistry , cell adhesion , viability assay , microbiology and biotechnology , traditional medicine , in vitro , biology , medicine , biochemistry , organic chemistry
Biodentine™ is relatively a new tricalcium silicate cement that has gained great attention of the researchers due to its biological potential in comparison with other materials. The aim of this study was to investigate the optimum concentrations of Biodentine in relation to its stimulatory or inhibitory effect on proliferation, migration and adhesion of stem cells of human exfoliated deciduous teeth (SHED). The cell cultures of SHED were treated with Biodentine™ extract at four different concentrations; 20mg/ml, 2mg/ml, 0.2mg/ml and 0.02mg/ml. Cells cultured without Biodentine™ were kept as a blank control. The proliferation potential of SHED cells was evaluated by MTT viability analysis for 6 days. Migration potential was investigated by wound healing and transwell migration assays. The growth, survival and communication potential of these cells was determined by Adhesion assay.