Open AccessAction of the natural compound gomisin a on Ca2+ movement in human prostate cancer cells
Author(s) -
Lyh-Jyh Hao,
Rong-An Lin,
LiChai Chen,
JueLong Wang,
I-Shu Chen,
Chun-Chi Kuo,
Chiang-Ting Chou,
Jau-Min Chien,
ChungRen Jan
Publication year - 2022
Publication title -
chinese journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.396
H-Index - 31
eISSN - 2666-0059
pISSN - 0304-4920
DOI - 10.4103/cjp.cjp_6_22
Subject(s) - thapsigargin , cytotoxicity , phorbol , pharmacology , apoptosis , chemistry , biochemistry , biology , signal transduction , protein kinase c , in vitro , endoplasmic reticulum
Gomisin A is a dietary lignan compound isolated from the fruit of Schisandra chinensis and has many pharmacological properties, including hepato-protective, anti-diabetic, and anti-oxidative activities. However, the benefit of gomisin A is still not well understood. The action of gomisin A is diverse. However, the effect of gomisin A on Ca 2+ signaling in prostate cancer cells is unknown. Ca 2+ is a pivotal second envoy that triggers and regulates cellular processes such as apoptosis, fertilization, energy transduction, secretion, and protein activation. The goal of this study was to explore the action of gomisin A on [Ca 2+ ] i and cytotoxicity in PC3 prostate cancer cells. Gomisin A at 100-200 μM provoked [Ca 2+ ] i raises. 20% of the response was reduced by removing external Ca 2+ . The Ca 2+ influx provoked by gomisin A was suppressed by 20% by store-caused Ca 2+ entry suppressors: econazole, SKF96365, nifedipine; also by phorbol 12-myristate 13 acetate and GF109203X. Without external Ca 2+ , gomisin A-caused [Ca 2+ ] i raises were abolished by thapsigargin. In contrast, gomisin A suppressed the [Ca 2+ ] i raises caused by thapsigargin. U73122 fell short to change gomisin A-caused [Ca 2+ ] i responses. Gomisin A (20-100 μM) elicited cytotoxicity in a dose-associated fashion. Blockade of [Ca 2+ ] elevations with 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid/acetoxy methyl failed to inhibit cytotoxicity of gomisin A. Collectively, gomisin A evoked [Ca 2+ ] i raises and provoked cytotoxicity in a Ca 2+ -dissociated fashion in prostate cancer cells.