Open AccessProzone phenomenon in pretransplant testing: An interesting conundrum involving solid-phase and cell-based assays
Author(s) -
Pranav Dorwal,
Parvind Singh,
AseemKumar Tiwari,
Vikash Chandra Mishra,
TruptiVimlakar Deshpande,
AmitKr Bhardwaj,
Shweta Kumari,
Vimarsh Raina
Publication year - 2022
Publication title -
asian journal of transfusion science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.262
H-Index - 22
eISSN - 1998-3565
pISSN - 0973-6247
DOI - 10.4103/ajts.ajts_145_20
Subject(s) - human leukocyte antigen , medicine , histocompatibility , antibody , immunology , antigen , histocompatibility testing , flow cytometry , panel reactive antibody , contraindication , transplantation , pathology , alternative medicine
Human leukocyte antigen (HLA) is a major determinant in deciding upon solid organ histocompatibility. Donor-specific anti-HLA antibodies (Donor-specific anti-HLA antibodies (DSAs)) are always a contraindication for solid organ transplantation, and identification of DSA becomes very crucial before transplantation to provide long-term graft survival. For identification of DSA, usually, either cell-based or HLA bead-based assay is being used in laboratories. However, both cell-based and bead-based assays have certain limitations. One such common limitation is "prozone effect," which can give false-negative results. Here, we would like to present a small pilot study to analyze the effect of the prozone phenomenon in the cell-based and HLA bead-based assays and its utility in histocompatibility testing.