Open AccessDirect modification of spermatogonial stem cells using lentivirus vectors in vivo leads to efficient generation of transgenic rats
Author(s) -
BangJin Kim,
Yong Hee Kim,
Myeong-Geun Oh,
KiJung Kim,
Sun Young Jung,
Ju-Hee Jin,
SunUk Kim,
KwanSik Min,
BuomYong Ryu
Publication year - 2019
Publication title -
asian journal of andrology/asian journal of andrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.701
H-Index - 74
eISSN - 1745-7262
pISSN - 1008-682X
DOI - 10.4103/aja.aja_80_18
Subject(s) - transgene , biology , germline , in vivo , genetically modified mouse , genetically modified organism , gene , microbiology and biotechnology , genetics
Spermatogonial stem cells (SSCs) transmit genetic information to the next progeny in males. Thus, SSCs are a potential target for germline modifications to generate transgenic animals. In this study, we report a technique for the generation of transgenic rats by in vivo manipulation of SSCs with a high success rate. SSCs in juvenile rats were transduced in vivo with high titers of lentivirus harboring enhanced green fluorescent protein and mated with wild-type females to create founder rats. These founder rats expressed the transgene and passed on the transgene with an overall success rate of 50.0%. Subsequent generations of progeny from the founder rats both expressed and passed on the transgene. Thus, direct modification of SSCs in juvenile rats is an effective means of generating transgenic rats through the male germline. This technology could be adapted to larger animals, in which existing methods for gene modification are inadequate or inapplicable, resulting in the generation of transgenic animals in a variety of species.