Open AccessCharacterization of Arachis hypogaea L. oil obtained from different extraction techniques and in vitro antioxidant potential of supercritical fluid extraction extract
Author(s) -
Rishika Chauhan,
Iftekhar Ahmad,
Yasmeen Khan,
Ennus Tajuddin Tamboli,
Sayeed Ahmad
Publication year - 2016
Publication title -
drug development and therapeutics
Language(s) - English
Resource type - Journals
eISSN - 2394-6555
pISSN - 2394-2002
DOI - 10.4103/2394-6555.191150
Subject(s) - arachis hypogaea , extraction (chemistry) , supercritical fluid extraction , chromatography , chemistry , supercritical fluid , in vitro , antioxidant , botany , biology , biochemistry , organic chemistry
Aim: The present investigation was aimed to characterize the fixed oil of Arachis hypogaea L. using five different extraction methods: Supercritical fluid extraction (SFE), ultrasound assistance extraction, soxhlet extraction, solvent extraction, and three phase partitioning method. Materials and Methods: The SFE conditions (temperature, pressure, and volume of CO 2) were optimized prior for better yield. The extracted oils were analyzed and compared for their physiochemical parameters, high-performance thin layer chromatography (HPTLC), gas chromatography-mass spectrometry (GC-MS), and Fourier transform infrared spectrometry (FT-IR) fingerprinting. Anti-oxidant activity was also determined using DPPH and superoxide scavenging method. Results: The main fatty acids were oleic, linoleic, palmitic, and stearic acids as obtained by GC-MS. HPTLC analysis revealed the presence of similar major components in chromatograms. Similarly, the pattern of peaks as obtained in FT-IR and GC-MS spectra of same oils by different extraction methods was superimposable. Conclusion: Analysis reported that the fixed oil of A. hypogaea L. is a good source of unsaturated fatty acid, mainly n-6 and n-9 fatty acid with a significant antioxidant activity of oil obtained from SFE extraction method