Open AccessSimultaneous determination of 18α-glycyrrhetinic acid and 18β-glycyrrhetinic acid in Glycyrrhiza glabra root by reversed phase high-performance liquid chromatography
Author(s) -
Ambika Chamoli,
Makhmur Ahmad,
Mohd. Rahil Hasan,
Bibhu Prasad Panda
Publication year - 2016
Publication title -
drug development and therapeutics
Language(s) - English
Resource type - Journals
eISSN - 2394-6555
pISSN - 2394-2002
DOI - 10.4103/2394-6555.180168
Subject(s) - glycyrrhiza , chromatography , chemistry , high performance liquid chromatography , reversed phase chromatography , phase (matter) , organic chemistry , medicine , alternative medicine , pathology
Background: The aim of the present research work is to develop a high-performance liquid chromatography (HPLC) method for simultaneous analysis of 18α-glycyrrhetinic acid (18α-GA) and 18β-GA (18β-GA) of Glycyrrhiza glabra. Materials and Methods: About 20 μL aliquots of each 18α-GA and 18β-GA were analyzed using reversed-phase C-18 column. The mobile phase was acetonitrile:tetrahydrofuran:water (10:80:10, v/v/v). The run time was 10 min at flow rate of 1 ml/min. Ultraviolet detection was carried out at 254 nm. Results: 18α-GA and 18β-GA were well resolved in reversed phase C-18 column using mobile phase acetonitrile: tetrahydrofuran: water (10:80:10, v/v/v, pH 7.9). The Rtof 18α-GA and 18β-GA was detected at 2.091 and 2.377 min, respectively. Conclusion: The developed chromatography method could be extended for potential quantification or simultaneous determination of these markers in plant as well as in herbal formulation