Open AccessHepatoprotective activity of Clearliv a polyherbal formulation in Wistar rats
Author(s) -
E.K. Dilip Kumar,
Vijay R Rajan,
Anil Kumar,
Subramani Parasuraman,
SF Emerson
Publication year - 2013
Publication title -
archives of medicine and health sciences
Language(s) - English
Resource type - Journals
eISSN - 2321-6085
pISSN - 2321-4848
DOI - 10.4103/2321-4848.123023
Subject(s) - thioacetamide , carbon tetrachloride , medicine , pharmacology , superoxide dismutase , liver injury , lipid peroxidation , glutathione peroxidase , glutathione , antioxidant , hydroxyproline , necrosis , malondialdehyde , catalase , oxidative stress , biochemistry , enzyme , chemistry , organic chemistry
Objective: To evaluate the hepatoprotective activity of Clearliv a polyherbal formulation in Wistar rats. Materials and Methods: The hepatoprotective potential of Clearliv was evaluated in thioacetamide-induced liver necrosis, DL-galactosamine [GalN]-induced liver injury, and carbon tetrachloride [CCl 4 ]-induced hepatitis models in Wistar rats. In all the models, Clearliv (at the dose levels of 800, and 1000 mg/kg) was administered for 3 days orally followed by single intraperitoneal administration of the hepatotoxicant on the last day after one hour of Clearliv administration. After 24 h of toxicant administration blood sample was collected by sino-orbital puncture in sodium EDTA tubes. The efficacy of Clearliv was evaluated by plasma biochemical parameters (AST, ALT, and ALP), and antioxidant enzyme (lipid peroxidation, catalase, superoxide dismutase, glutathione peroxidase, and hydroxyproline) levels. Results: In thioacetamide-induced necrosis, and GalN-induced liver injury models, Clearliv at 1000 mg/kg showed significant reduction in the elevated plasma liver markers, and elevated antioxidants levels. In CCl 4 -induced hepatitis, the Clearliv had favorable hepatoprotective effect, but the results were not significant. Conclusion: Clearliv 800, and 1000 mg/kg showed significant hepatoprotective effect against thioacetamide- and GalN-induced liver necrosis and injury, respectively