Open AccessPhenotypic characterization ofNocardiaspp. isolated from Iran soil microflora
Author(s) -
Shadi Habibnia,
Masoumeh Rasouli Nasab,
Parvin Heidarieh,
Mehdi Fatahi Bafghi,
Mohammad Reza Pourmand,
Seyyed Saeed Eshraghi
Publication year - 2015
Publication title -
international journal of environmental health engineering
Language(s) - English
Resource type - Journals
ISSN - 2277-9183
DOI - 10.4103/2277-9183.158388
Subject(s) - nocardia , biology , nocardiosis , nocardia infections , microbiology and biotechnology , actinomycetales , nocardiaceae , streptomyces , bacteria , genetics
Aims: The present study was conducted to identify Nocardia spp. from Iran soil by various phenotypic tests. Materials and Methods: A total of 300 soil samples were collected of five different geographical regions in Iran. Nocardia isolation was performed by paraffin baiting technique. The colonies that were similar to be Nocardia spp. were stained with Gram, partially acid fast and acid-fast. Phenotypic tests were used for identification of Nocardia spp. Results: After analysis of phenotypic tests, the identified species are as follow: Nocardia asteroides (49.12%), Nocardia cyriacigeorgica (24.56%), Nocardia otitidiscaviarum (38.6%), N. asteroides complex (19.29%), Nocardia africana (3.5%), and Nocardia pseudobrasiliensis, Nocardia coubleae, Nocardia ignorata (each species; 1.75%). In this study, some of isolates (8.77%) remained undetected.Conclusion: Due to great number of Nocardia spp., high similarities among biochemical characteristics of species and the variability of some these characters, a wide range of biochemical tests should be used to identify Nocardia spp. to gain more accurate results