Open AccessFabrication and characterization of biodegradable polymeric films as a corneal stroma substitute
Author(s) -
Sahar Salehi,
Mohammadhossein Fathi,
Shaghayegh Haghjooy Javanmard,
Farnaz Barneh,
Mona Moshayedi
Publication year - 2015
Publication title -
advanced biomedical research
Language(s) - English
Resource type - Journals
ISSN - 2277-9175
DOI - 10.4103/2277-9175.148291
Subject(s) - sebacic acid , glycerol , fourier transform infrared spectroscopy , viability assay , materials science , tissue engineering , cytotoxicity , biodegradable polymer , in vitro , nuclear chemistry , polymer , chemistry , biomedical engineering , chemical engineering , polymer chemistry , organic chemistry , biochemistry , medicine , engineering
Background: Biodegradable elastomeric materials such as poly glycerol sebacate (PGS) have gained much current attention in the field of soft tissue engineering. The present study reports the synthesis of PGS with molar ratios of 1:1, 2:3, and 3:2 of glycerol and sebacic acid via polycondensation reaction and tests the effect of PGS on human corneal epithelial (HCE) cells viability in vitro. Materials and Methods: PGS films were prepared by the casting method. We tried to fabricate PGS with different compositions and various properties as being a viable alternative to the corneal stroma in cornea tissue engineering. The chemical properties of the prepared polymer were investigated by means of attenuated total reflectance - Fourier transform infrared spectroscopy (ATR-FTIR) analysis and the in vitro cytotoxicity was investigated by the Alamarblue method. Results: The functional groups observed in the PGS FTIR spectrums of PGS with various molar ratios were the same. However, the main difference was the time of completing the cross-linking reaction. The PGS prepared by 2:3 ratio as a molar ratio had the fastest and the 3:2 ratio had the lowest cross-linking rate because of the higher amount of sebacic acid. Results of the Alamarblue cytotoxicity test assay showed no deleterious effect on HCE cell viability and proliferation.Conclusions: PGS is a potentially good candidate material for corneal tissue engineering because of its lack of in vitro HCE cell toxicity