Open AccessComparison of three phenotypic and deoxyribonucleic acid extraction methods for isolation and Identification of Nocardia spp
Author(s) -
Jamshid Faghri,
Samane Bourbour,
Sharare Moghim,
Mohsen Meidani,
Hajiye Ghasemian Safaei,
Nasrin Hosseini,
Bahram Nasr Esfahani,
Hussein Fazeli,
Mansour Sedighi
Publication year - 2014
Publication title -
advanced biomedical research
Language(s) - English
Resource type - Journals
ISSN - 2277-9175
DOI - 10.4103/2277-9175.137839
Subject(s) - dna extraction , nocardia , dna , isolation (microbiology) , bacteria , biology , genotyping , microbiology and biotechnology , polymerase chain reaction , genotype , genetics , gene
Background: The aerobic Actinomycetes are a large group of soil-indwelling bacteria that are distributed in world-wide. These Gram-positive bacteria are most commonly associated with opportunistic infections in both immunocompromised and immunocompetent hosts. Materials and Methods: In this study, three phenotypic and deoxyribonucleic acid (DNA) extraction methods for isolation and identification of Nocardia genus were compared. Samples were taken in five different locations of Isfahan′s suburb from hospitals area, parks, agricultural lands, gardens, arid lands with different soil temperature and pH. Results: In this study, showed that slip-buried-method was better than two other phenotypic methods; 14 out of 70 soil samples (20%) were positive for Nocardia spp. DNA of positive samples were extracted with three techniques and DNA extraction by microwave technique was better than others. This technique was confirmed with observation of DNA bands on 1% agarose gel.Conclusions: These bacteria are important in immune deficient patients such as cancer patients, transplant recipients, tuberculosis; acquired immunodeficiency syndrome etc., Their affluence is unsteady in different zones of the world. In this study, among the three phenotypic methods for the isolation of Nocardia slip-buried method was better than other methods. Among DNA extraction techniques, DNA extraction by microwave method would be selective method for DNA extraction of Nocardia spp. compared with others techniques