Open AccessA reverse phased high-pressure liquid chromatographic method for the estimation of a poisonous matter in Strychnos nux-vomica
Author(s) -
Achu Hashim,
Rafique Mohammed,
Dilshad Umar,
Vaswani R Veena,
Basheer Bahija,
Kusai Baroudi
Publication year - 2015
Publication title -
journal of advanced pharmaceutical technology and research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.325
H-Index - 33
eISSN - 2231-4040
pISSN - 0976-2094
DOI - 10.4103/2231-4040.161506
Subject(s) - chromatography , strychnos , chemistry , thin layer chromatography , phytochemical , high performance liquid chromatography , strychnine , toluene , crude drug , solvent , alkaloid , organic chemistry , biochemistry
Seeds of Strychnos nux-vomica were subjected to preliminary phytochemical tests and its presence was confirmed by thin layer chromatography (TLC) method. The TLC profile of the methanolic extract of seeds of S. nux-vomica was developed using the solvent system toluene:chloroform:methanol in the ratio 8:2:1. The plate was observed in visible light after spraying with Dragendorff's reagent (specific method). High-performance liquid chromatography (HPLC) profile of the methanolic extracts of S. nux-vomica was developed, and the amount of strychnine seems to be 0.36% (w/w) in the seeds. The TLC and HPLC profiles developed are very valuable for the identification of the original drug from their adulterants. The TLC profile identifies the presence of strychnine in the plant material. The quantification method for the strychnine in the seeds can be used for the quality standardization of the raw drug because the strychnine is reported to have some toxicity.