Open AccessAn in vitro investigation of a newer intracanal medicament Nisin on Enterococcus faecalis in comparison with chlorhexidine and calcium hydroxide
Author(s) -
Chinni Suneelkumar,
Ashok Baskaran Veni,
Manali Ramakrishnan Srinivasan,
Rajamani Indra
Publication year - 2011
Publication title -
journal of international clinical dental research organization
Language(s) - English
Resource type - Journals
eISSN - 2231-5357
pISSN - 2231-0754
DOI - 10.4103/2231-0754.115770
Subject(s) - enterococcus faecalis , calcium hydroxide , chlorhexidine , nisin , root canal , dentin , dentistry , endodontics , chemistry , medicine , microbiology and biotechnology , staphylococcus aureus , biology , antimicrobial , bacteria , genetics
Aim: To determine the antibacterial efficacy of Nisin in comparison with Calcium hydroxide and Chlorhexidine. Materials and Methods: Fifty extracted lower premolar single-rooted teeth were collected and were instrumented with K3 rotary files. Then, root canals were inoculated with a bacterial solution of Enterococcus faecalis. After 21 days, the canals were inoculated with Saline, Nisin, Vancomycin, Calcium hydroxide, and Chlorhexidine. The roots were left for 7 days and on the 8 th day, to investigate the degree of infection of the radicular dentin, specimens of the dentin chips from the full length of the root canal were harvested using a sterile rotary K3 instrument (size 25 6% taper). Results: The results of the present study showed that Nisin and Chlorhexidine showed none of Colony Forming Units (CFU) in their respective group. Conclusion: Within the limits of the study, Nisin was effective in eradicating E. faecalis cells in pure culture and root canal dentin