Open AccessComparison of protein expression pattern between the Plasmodium falciparum chloroquine-resistant RKL9 and chloroquine-sensitive MRC2 strains
Author(s) -
Hiasindh Ashmi Antony,
Vrushali Pathak,
Kanjaksha Ghosh,
Subhash Chandra Parija
Publication year - 2016
Publication title -
tropical parasitology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.418
H-Index - 11
eISSN - 2229-7758
pISSN - 2229-5070
DOI - 10.4103/2229-5070.190831
Subject(s) - strain (injury) , plasmodium falciparum , two dimensional gel electrophoresis , microbiology and biotechnology , spots , biology , extracellular , isoelectric focusing , intracellular , gel electrophoresis , chloroquine , biochemistry , proteomics , gene , enzyme , malaria , immunology , botany , anatomy
The objective of this study was to compare the protein expression patterns of Plasmodium falciparum extracellular and intracellular proteins separated by two-dimensional electrophoresis (2-DE) from the chloroquine-sensitive (CQS) MRC2 strain and chloroquine-resistant (CQR) RKL9 strain. Materials and Methods: Both the extracellular protein (ECP) and intracellular protein (ICP) were extracted and solubilized. The proteins were separated by 2-DE, first based on their charges using isoelectric focusing and then their sizes by electrophoresis. The separated protein spots were detected by silver staining, and further, the protein spot density was analyzed by an image analysis software.