Open AccessComparative evaluation of staining techniques and polymerase chain reaction for diagnosis of intestinal microsporidiosis in immunocompromised patients
Author(s) -
Ujjala Ghoshal,
Sonali Khanduja,
Vikas Agarwal,
T.N. Dhole,
Uday C Ghoshal
Publication year - 2015
Publication title -
tropical parasitology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.418
H-Index - 11
eISSN - 2229-7758
pISSN - 2229-5070
DOI - 10.4103/2229-5070.162491
Subject(s) - microsporidiosis , polymerase chain reaction , staining , pathology , biology , medicine , microsporidia , microbiology and biotechnology , genetics , gene , spore
Microsporidia, which causes chronic diarrhoea in immunocompromised hosts, are often missed. The commonest diagnostic techniques include modified trichrome (MT) stain; however, it requires expertise and does not identify the species, which is important therapeutically. Other diagnostic techniques include Calcoflour white staining and polymerase chain reaction (PCR). Data on comparative utility of different diagnostic techniques are scanty.