Open AccessLaboratory methods of identification of Entamoeba histolytica and its differentiation from look-alike Entamoeba spp.
Author(s) -
Subhash Chandra Parija,
Jharna Mandal,
Dinoop Korol Ponnambath
Publication year - 2014
Publication title -
tropical parasitology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.418
H-Index - 11
eISSN - 2229-7758
pISSN - 2229-5070
DOI - 10.4103/2229-5070.138535
Subject(s) - entamoeba histolytica , entamoeba , entamoeba coli , amoebiasis , biology , microbiology and biotechnology , identification (biology) , immunology , helminths , botany , ascaris lumbricoides
Entamoeba histolytica, the causative agent of intestinal and extraintestinal amebiasis, is a common parasitic cause of significant morbidity and mortality in the developing countries. Hence, early detection and differentiation of pathogenic E. histolytica from nonpathogenic/commensal Entamoeba spp (Entamoeba dispar/Entamoeba moshkovskii/Entamoeba bangladeshi) plays a crucial role in clinical management of patients with amebiasis. Most diagnostic tests currently available do not reliably differentiate between the species of Entamoeba and are less sensitive, cumbersome to perform. Molecular-based methods are highly sensitive, easy to perform and differentiates the pathogenic Entamoeba from nonpathogenic species, serving the criteria for an ideal diagnostic test for amebiasis. Recently, microarray technology has been found to be a promising tool for the diagnostic and epidemiological evaluation of amebiasis.