Analysis of a novel multiplex polymerase chain reaction assay as a sensitive tool for the diagnosis of indeterminate and tuberculoid forms of leprosy | Zendy

V.S. Chaitanya | Zendy; Luz Cuello | Zendy; Madhusmita Das | Zendy; A Sudharsan | Zendy; Pavithrra Ganesan | Zendy; K. Kanmani | Zendy; Lakshmi Rajan | Zendy; Mannam Ebenezer | Zendy

Open Access

Analysis of a novel multiplex polymerase chain reaction assay as a sensitive tool for the diagnosis of indeterminate and tuberculoid forms of leprosy

Author(s) -

V.S. Chaitanya,

Luz Cuello,

Madhusmita Das,

A Sudharsan,

Pavithrra Ganesan,

K. Kanmani,

Lakshmi Rajan,

Mannam Ebenezer

Publication year - 2017

Publication title -

international journal of mycobacteriology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.53

H-Index - 20

eISSN - 2212-554X

pISSN - 2212-5531

DOI - 10.4103/2212-5531.201885

Subject(s) - leprosy , mycobacterium leprae , multiplex polymerase chain reaction , polymerase chain reaction , multiplex , indeterminate , lepromatous leprosy , tuberculoid leprosy , medicine , biology , pathology , gene , genetics , mathematics , pure mathematics

Clinical diagnosis of indeterminate and tuberculoid leprosy is often difficult due to limited and confounding signs and symptoms. In the current study, we evaluated the utility of new multiplex polymerase chain reaction (PCR) using Mycobacterium leprae-specific DNA sequences in the pseudogene regions of ML1545, ML2180, and ML2179 for PCR-based diagnosis of indeterminate leprosy (IND) and leprosy cases across the immunological spectrum. The sensitivity was compared with that of RLEP PCR.

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